Atividade antimicrobiana e antibiofilme da própolis vermelha e da resina de Amburana cearensis sobre Staphylococcus coagulase positiva causadores de mastite subclinica em vacas

Abstract

The objective of this study was to evaluate the antimicrobial and antibiofilm activity of red propolis and Amburana Cearensis resin on SCP that cause subclinical mastitis, isolated from bovine milk samples from dairy herds in the states of Alagoas and Pernambuco. Fourteen properties were selected, 10 located in municipalities in the dairy basin of the State of Alagoas and 4 in municipalities in the dairy basin of the State of Pernambuco, which supplied milk for dairy under federal inspection. The CMT test was performed in 1405 cows and positive CMT samples from 2+ were collected, totaling 1289 samples submitted to microbiological examination for SCP isolation and identification, totaling 179 isolates. SCP were submitted to the standard antibiotic (Oxacillin) sensitivity test, by the disk diffusion method and the resistant isolates were submitted to the microplate adhesion test to quantify the biofilm. In the SCP isolates, classified as strong and moderate biofilm formers, the search for nuc, blaZ, mecA, mecC, icaA and icaD genes was carried out and the antimicrobial and antibiofilm activity of red propolis and Amburana Cearenses resin was evaluated by microdilution method in broth, being carried out the determination of Inhibitory Concentrations (MIC) and Bactericidal (CBM) of the substances and the antibiofilm action. MIC values ranged from 3.125 to 781.25 μg/mL for red propolis and from 195.31 to 390.62 μg/mL for umburana resin. The red propolis presented a CBM ranging from 3,125 to 1,565.50 μg/mL, while the CBM for the umburan resin ranged from 195.31 to 781.25 μg/mL. Both substances were also effective in reducing the formation and consolidation of biofilms, suggesting a promising action of these substances against this structure of microbial resistance and being a possible alternative for their future use as drugs with antimicrobial action.