Avaliação da atividade antibacteriana de plantas medicinais com indicação antidiarreicas contra sorotipos de Escherichia coli

Abstract

Knowledge about medicinal plants is always going to be really important in the process of obtaining data for the microbiological and pharmacological study of species. In order to alleviate or cure diseases, we use plants as therapeutic options. These may be used to treat basic human diseases such as diarrhea, which is one of the major concerns due to contamination by Escherichia coli (E. coli). Therefore, the objective of this work was to prove the antibacterial activity of medicinal plants traditionally used in the treatment of intestinal disorders. The plants used for the treatment of diarrhea were Anadenanthera colubrina (Vell.) Brenan and Carica papaya L., they were selected through an ethnodirected research. Then the aqueous extracts were prepared for chemical and microbiological tests. The chemical constituents will be revealed by the HPLC-ESI-MS technique. In vitro microbiological analyzes were performed to determine the Minimum Inhibitory Concentration (CIM) and the antibiotic modulating activity evaluation were performed using the broth microdilution methodology, using the antibiotics like ciprofloxacin, trimethoprim sulfamethoxazole and metronidazole. The bacterial strains were enteropathogenic E. coli (EPEC) and enterotoxigenic E. coli (ETEC). The statistical analyzes of microbiological results were performed using software known as Graphpad Prism 7.0, followed by Bonferroni post-test with p <0.05 for significant results. It is concluded from this work that the use of vegetables, or their combination with antibacterial drugs, do not offer an alternative to combat diarrheal diseases caused by EPEC and ETEC serotypes of E. coli.