Avaliação do efeito da ozonioterapia e cisplatina em modelo experimental de camundongos com carcinoma de Ehrlich.

Abstract

Neoplasms represent one of the main causes of death in humans and animals, it is a complex disease characterized by permanent modification of the cell's genetic material. Ozone therapy is described as a therapeutic approach for the coadjutant treatment of cancer and side effects associated with chemotherapy and radiotherapy. This paper aims to evaluate albino Swiss mice (Mus muscullus) with Ehrlich carcinoma submitted to treatments with intrarectal ozone therapy and cisplatin. The experiment consisted of using 25 Swiss albino mice (Mus muscullus), females with approximately 60 days old, with body weight between 35g and 40g, inoculated with Ehrlich carcinoma, divided into four groups, Group G1: Positive control: 2.5mg / kg of Cisplatin; Group G2: Ozone-Oxygen Mixture via Rectal Insufflation; Group G3: 2.5mg / kg of Cisplatin + Ozone-Oxygen Mixture via Rectal Insufflation and Group G4: Negative control 1ml of 0.9% saline solution. Animals in all groups underwent an adaptation period of eight days, in which groups G2 and G3 were subjected to ozone therapy via rectal insufflation for six days. After this period, Ehrlich carcinoma cells were inoculated in all animals. After 48h of the inoculation, the different treatments were started for six days, at the end of the experiment the animals were euthanized, blood was collected through cardiac puncture, and the tumor and organs were removed (lung, spleen, kidneys and liver). The results related to the blood count, renal and hepatic profile did not show statistically significant differences (p <0.05) between the groups for the analyzed variables. The weight of the tumor and each of the organs analyzed (Lung, Spleen, Kidney, Liver) showed statistically significant differences between the groups. For these variables, it is highlighted that the tumor average weight was higher in the G4 group (3.83 g), it was lower in the G3 group (0.79 g). And these significant differences were noted between the negative control group with the other groups. In the histopathological exam, there were no statistically significant differences (p <0.05) between groups. It is concluded that, according to the results, ozone therapy associated with cisplatin proved to be the treatment in which mice with Erhlich carcinoma had the lighter tumor weight. The therapeutic actions in the group treated only with ozone therapy provided an increase in food consumption, as well as an antitumor activity similar to the group treated only with cisplatin.