Avaliação morfológica, morfométrica, histopatológica e termográfica de retalhos cutâneos tratados com pomada fitoterápica de Anthurium affine schott em ratos

Abstract

The objective of this work was to evaluate, by infrared thermography, morphometry and macro and microscopic morphology, the healing activity of the ointment composed of hydroalcoholic extract of Anthurium affine Schott in dorsal cranial-based random cutaneous flaps in male Wistar rats (n=18), with 90 days of age and randomly divided into three groups: Absolute Control Group (CAG) – animals without treatment (n=06); Control Group (GC) - animals treated with the ointment base (n=6); Treated Group (TG) – animals treated with A. affine Schott ointment (n=6). Each animal was submitted to the making of 8 cm long by 2 cm wide skin flaps. The GC and GT received treatment every 24 hours for 7 days. All groups were evaluated with thermography after shaving, after surgery and every 24 hours until the eighth day, when they were euthanized. On the last day of the experiment, the wounds were measured with a digital caliper to assess the area of necrosis. Histological sections of the cervicothoracic, thoracolumbar and lumbosacral portions of the flaps were stained with Hematoxylin-Eosin and Trichrome of Gômori for morphometric and morphological analysis by quantifying blood vessels and determining collagen structuring. The data obtained were submitted to the ANOVA test complemented by the Tukey test (p<0.05). Infrared thermography proved to be a promising method for the evaluation of areas of skin flap necrosis. Treatment with ointment containing hydroalcoholic extract of A. affine Schott did not favor the healing process of the dorsal skin flaps. However, we emphasize that further studies are needed with different concentrations of the extract.