Bioatividade de micropartículas de quitosana contendo trans-cinamaldeído contra Staphylococcus aureus resistente a Meticilina (MRSA)

Abstract

The occurrence and spread of bacterial resistance (RAM) has been identified as a public health problem, and one of the main ways of spreading these resistant bacteria is the lack of hygienic-sanitary practices in the food storage processing environment. The aim of the present work was to produce chitosan microparticles containing trans-cinnamaldehyde with bioactivity in the inhibition of Staphylococcus aureus MRSA. Chitosan microparticles containing trans-cinnamaldehyde were prepared using the simple coacervation technique, using 5% (w / v) chitosan as the wall material and vanillin as the crosslinking material. The characterization was performed in terms of encapsulation efficiency, average microparticle diameter, polydispersity index, zeta potential, morphology through MEV and optical microscopy. The antibacterial analysis of the particles against Staphylococcus aureus MRSA was performed using a broth microdilution technique, determining the Minimum Inhibitory Concentration (MIC). The microparticles had an average diameter of 1.92 ± 0.005 mm, a PDI of 0.39 ± 0.2, a surface load of 20.61 ± 0.8 mV, an encapsulation efficiency of 93.81% and a shape spherical and wrinkled. The antibacterial activity tests showed that the chitosan microparticles containing the trans-cinnamaldehyde showed antibacterial activity for strains of S.aureus ATCC (6538) as well as for strains S. aureus MRSA, obtaining lower MIC values than those obtained for treatments with free oil and empty microparticles. Thus, chitosan microparticles containing trans-cinnamaldehyde may be a promising alternative for controlling the multiplication and dissemination of methicillin-sensitive and resistant S. aureus (MSSA and MRSA).