Avaliação de antígenos do vírus da artrite encefalite caprina obtidos a partir de células bovinas (CFBov) e sua aplicação na Imunodifusão em Gel de Agar (IDGA)

Abstract

The immunodiagnosis applied to the identification of caprine arthritis encephalitis (CAE) is considered the best alternative for the prevention and control of infections, enabling the detection of infected animals at an early stage. The production process of the antigens is fundamental for the performance of this test, which makes the development of research contributing to obtaining it, extremely important. The objective of this research was to produce viral antigen through bovine fibroblastic cells (CFBov) infected with the caprine arthritis encephalitis virus (CAEV) for use in IDGA tests, without previous treatment and treated with Sodium Duodecyl Sulfate (SDS) detergents, Triton X-100 and Tween 20. 270 serum samples obtained from the serum bank of the Animal Virology Laboratory (LAVIAN) of the Federal Rural University of Pernambuco, from goats from local herds, seronegative and seropositive tested for CAEV, were used. When analyzing the results, it was found that 38 samples were positive (14.07%) and 232 negative (85.92%) when tested with pure antigen without any treatment; 39 samples were positive (14.44%) and 231 negative (85.55%) when treated with the SDS detergent; 35 samples were positive (12.96%) and 235 negative (87.03%) when treated with Triton X-100 detergent; and 36 samples were positive (13.33%) and 234 negative (86.66%) when treated with the Tween 20 detergent. The sensitivity and specificity for each treatment was calculated and the tests reached relating to commercial antigen respectively, 94.7% and 99.6% for pure antigen; 97.4% and 98.7% for SDS; 97.2% and 98.7% for Triton X-100; and, 97.3% and 99.1% for Tween 20, with SDS being the most efficient. The kappa coeficiente was 1.0. The production of antigens from bovine cells and their purification using SDS as a detergent, proved to be a high quality and effective alternative for the diagnosis of CAE.