Avaliação dos parâmetros fisiológicos, hematológicos e dosagens de mediadores biológicos em cadelas submetidas à ovariosalpingohisterectomia após a administração de sangue medular

Abstract

This work aimed to evaluate the inflammatory and pain response after bone marrow application preoperatively in female dogs undergoing ovariosalpingohysterectomy, measuring the cytokines intereukin-6, tumor necrosis factor alpha, transforming growth factor beta, myelogram, physiologic parameters and serum cortisol. Thirty female dogs, clinically healthy, from different breeds, with an average weight of 13,76 kg, from the veterinarian hospital of Universidade Estadual do Maranhão, distributed randomly and equally into three groups: GI (Control), GII (Conventional) e GIII (Experimental).GI received analgesia with tramadol, GII with benzatinpenicilina 40.000 UI/kg and GII received tramadol plus 10 ml of autologous bone marrow, collected from humerus proximal metaphysis before the surgery, and applied on biceps femoris muscle. 5 ml of blood were collected for cytokines measurements, in five different times: m0 (before surgery), M1 (6 hours after surgery), M2 (48 hours), M3 (120 hours) and M4 (168 hours). Samples with 0,5 ml of blood were collected for myelogram. Interleukin-6 showed different statistical significance between GIII lowest average and GII highest average. Tumor necrosis factor alpha evaluation showed significant difference between GIII and GI. Transforming growth factor beta analysis showed differences between GIII, with the lowest average on GII. The myelogram did not exhibited differences among groups. There were no differences relating to physiologic parameters. Regarding cortisol levels, the experimental group had inferior values comparing to the others. It is concluded that the bone marrow contributes to surgery recovery of dogs underwent ovariosalpingohysterectomy and that the pro and anti-inflammatory cytokine production remained balanced.