Bioensaios e estudo da atividade anti-inflamatória da semente de sucupira branca (Pterodon emarginatus, Vogel), utilizando camundongos em modelo experimental de osteoartrite

Abstract

The sucupira is a large canopy tree that folk medicine used as an alternative treatment for various diseases, these are known to the actions of the essential oil extracted from the leaves, stem bark, root or tuber, but the use of his seed is still rarely discussed in the scientific community. Aiming to determine the potential anti-inflammatory, analgesic potential, their minimum amount necessary to establish its action, toxicity and induction in the formation of fibroblasts, this experiment was conducted in the laboratory of Pharmacology UFRPE with six groups of mice receiving the fraction organic extract sucupira white (FOESSB) at different doses on alternate days, where it was possible to determine the dose and work with other groups of mice were pre-treated with some drugs and the FOESSB by simulation of inflammatory arthritis. Bioassays of spontaneous activity revealed that FOESSB provided a list of behavioral responses characteristic signaling and stimulating effects on the CNS depressant effects on the ANS and other miscellaneous purposes, though, the dose effect relationships found in responses associated with important CNS effects, it is recommended for further studies are employed doses lower than 385.71 mg / kg. The FOESSB, the dose reduced inflammation significantly, did not significantly change in test anxiety, reduced pro-inflammatory responses associated with the process of joint incapacitation induced by ovalbumin, significantly reduced the changes in the excitability threshold responses painful in the hot plate model 55oC and biochemical evaluations of parameters AST, ALT, ALP, urea and creatinine revealed that the antigenic stimulus had and probably effective participation in the rise of all these parameters, except for the levels of urea that were well below expectations in relation to the reference values in the literature for the same parameters in healthy Swiss mice and finally, there were no significant differences in macroscopic evaluation and weighing performed on organs of mice and removed after euthanasia.