Análise epidemiológica da infecção por Mycoplasma bovigenitalium e Ureaplasma diversum em bovinos na microrregião do Vale do Ipanema - Pernambuco

Abstract

This study’s objective was to conduct a Mycoplasma bovigenitalium and Ureaplasma diversum infections epidemiological investigation in cattle in the Ipanema Valley microregion, Pernambuco state. Vaginal swabs were collected from 355 reproductive age cows and submitted to polymerase chain reaction (PCR) and microbiological isolation. An investigative questionnaire was used to risk factors analysis associated with Mollicutes infection. According to PCR, 9.29% (33/355) of the samples were positive for Mycoplasma bovigenitalium and 21.69% (77/355) for Ureaplasma diversum. Coinfection was observed in 2.81% (10/355) of the samples. According to isolation, there was growth on Hayflick medium of 81.81% (27/33) from the samples classified as Mycoplasma spp. and 24.67% (19/77) growth on UB medium, being classified as Ureaplasma diversum. Risk factors associated to Mollicutes infection were considered: semi-intensive breeding system (OR = 4.6), pasture rent (OR = 3.6), reproductive disorders nonisolated animals (OR = 3.2), and natural mating plus artificial insemination (OR = 3.5). This is the first record of Mycoplasma bovigenitalium and Ureaplasma diversum infection in semiarid region cattle herds of Pernambuco state, Brazil. From this, preventive measures directed to the identified risk factors can contribute to Mollicutes’ occurrence decrease in the cattle herds studied.