Uso de miricetina em meio de criopreservação de sêmen ovino

Abstract

Ram semen was diluted (200 x 106 sperm/mL) in Tris-egg yolk (5% glycerol), added of myicetin (0.1, 10, 20, 30, 40, 100, 200, 300, 400 and 1000 nM) and frozen (-196 ° C). After thawing (37°C/30s), kinetics, iMPA, PMM, iROS, LPO, eMP and fertility in vivo (0 and 100 nM myricetin) were evaluated. The analyzes of 0, 1, 10, 100 and 1000 nM of myricetin showed: 10nM myricetin showed lower % RAP (P≤0.05), than 1000nM. Samples of control showed higher (P≤0.05) VAP than 10nM, while samples with 10, 100 and 1000nM of myicetin showed higher BCF (P≤0.05), when compared to control. Miricetin 1000nM presented higher percentage (P <0.05) of cells with LPO, than the control. For 0, 20, 30, 40, 100, 200, 300 and 400 here was no treatment effect (P>0.05) for kinetics and flow cytometry (P> 0.05), there were also no interactions between treatment and time (P> 0.05). For both there was effect of the incubation time, in some evaluated parameters, the PM, VCL, VSL, VAP, LIN, ALH, percentage of cells with intact plasma and acrosomal membrane, oxidative stress and membrane stability were reduced (P <0.05) in all groups, while BCF (P <0.05) increased in all groups.. The pregnancy rate in the 100 nM myricetin group was 50% (5/10) and that of the 10% control (1/10). In the in vitro analysis, it was concluded that myricetin has no antioxidant effect on cryopreserved spermatozoa. However, it improves the pregnancy rate of inseminated sheep.