Efeitos do inibidor de fosfodiesterase-5 (Tadalafil) em regeneração testicular após estresse térmico

Abstract

PDE inhibitors have been used for the treatment of erectile dysfunction, but their therapeutic potential is still unknown and little explored, among them Tadalafil, its daily use has been investigated in clinical trials and demonstrated to be a therapy capable of inhibiting inflammatory chains, the analysis of the heat applied directly to the testis has provided new information on the triggering mechanisms of inflammation and possible heat damage to tissues. The objective of this study was to evaluate the effect of tadalafil on the recovery of the testicular parenchyma of male Wistar rats, submitted to testicular thermal stress, for which 54 Wistar rats were used. Randomly distributed in 3 groups, where the control group suffered thermal insult and treated with intraperitoneal saline and the group treated with tadalafil at a daily intraperitoneal dose of 0.9 mg / kg-1 and 1.8 mg / kg-1. These animals were submitted to the same experimental condition, submitted to euthanasia procedures and perfused intraperitoneally. Oxidative stress of liver and spleen, cytokines IL-6 and TNF-α of splenic acid, plasma testosterone levels and histopathological analysis of the testis were measured. Body or testicular weight was not influenced by the administration of tadalafil, but was effective in reducing plasma testosterone levels on all evaluated days, also responsible for inhibiting the formation of NO in the liver and spleen. Low-dose Tadalafil exerted an anti-inflammatory effect on the levels of TNF-α (decreasing) and IL-6 (increase) in the splenic parenchyma during the experimental period. Tadalafil delayed the thermal stress lesions in the 7-day group with the two doses applied, and 15 days with a dose of 0.9 m / kg post-stress in the testicular parenchyma of rats, evidenced on histopathological analysis.