Avaliação do processo de descelularização de tecido cutâneo canino por método simples e conjugado

Abstract

This study aimed to assess the feasibility of the decellularization process in canine skin tissue using a simple method (dehydration glycerin) and conjugate (supersaturated sugar solution followed by dehydration of glycerin). After the preparation of supersaturated solution of sugar 300%, and departed a rate for physico - chemical and microbiological. Divided a skin fragment canine in 54 samples, distributed into 2 groups: 27 samples in group A (supersaturated sugar solution) and 27 samples in the group G (glycerin). Before the split, the fragment was subjected to microbiological evaluation. The storage temperature was 4 ° C. Subdivided into 6 study groups, each of 9 fragments. Subgroups A (A1, A2, A3) and subgroups G (G1, G2, G3). After 48 hours of immersion, each sample group were removed from the medium and subjected to microbiological evaluation new. The A1 group was immediately subjected to histological analysis. The other groups (A2 and A3) were transferred to glycerin and remained immersed for 13 days (A2) and 28 days (A3). Samples of the group G were taken from among the following chronology: G1 48 hours, G2 and G3 15 days with 30 days for histological evaluation. Changes in the morphology of collagen fibers and presence of cellular components in the epidermis and dermis ranked the treatments effective, effective with changes in collagen fibers and not effective. The physicochemical analysis of the supersaturated solution obtained pH 8.05, 25% moisture content and water activity 0.8487. With regard to microbiological evaluation of the solution, it was found absence of any microbial growth, as the skin was positive for Staphylococcus aureus. Only one (3.73%) The skin remained contaminated groups. A (11.11%) of the skin fragments group A1 was considered effective in decellularizing process change in the morphology of the collagen fibers. 88.88% of group A2 fragments presented decellularization the superficial and deep dermis with mild and moderate presence of cells. A (11.11%) of group A2 fragments decellularization was moderate in the epidermis. The group A3 was obtained the same results of the A2 group. With respect to the classification of groups G, the results were similar to group A at the same timing. It follows that the supersaturated sugar solution has antimicrobial action 48 hours of immersion and that both the supersaturated sugar solution as glycerin is not feasible to promote complete decellularization in canine cutaneous tissue.