Vírus da síndrome da mancha branca (WSSV) em crustáceos e moluscos nativos no Rio Paraíba - PB

Abstract

The expansion of shrimp farming in Brazil, especially in the Northeast, undergoes a change in the management environment, with the Pacific White shrimp, Litopenaeus vannamei, being cultured in freshwater, whose health challenges need to be dimensioned. In this sense, we applied the Loop Mediated Isothermal Amplification (LAMP) technology in order to investigate the possibility of reservoirs or carrier of Whit Spot Syndrome Virus (WSSV) in Paraíba River, in Paraíba Valley – PB. Were selected eight (8) sampling points on the river, near sources of water used by shrimp farms of the species L. vannamei. At each point, 10 specimens of Paleomon pandaliformis, 10 Pomacea lineata, and 10 Melanoides tuberculatus, were collected. These animals were stunned by thermal shock and shrimps had the nerve cord cut, in order to proceed with the collection for LAMP use. Gills fragments were removed from the shrimps, foot muscles fragments from P. lineata and the species M. tuberculatus were collected entire and all samples were stored in microcentrifuge tubes with 95% ethanol (1:10) .The analyses were performed in the laboratory of molecular analyses of Potiporã - RN and all samples were submitted to LAMP (LAMP Kit WSSV, Concepto Azul, Ecuador) for detection of WSSV. In the end, 81 samples of P. pandaliformis, 40 samples of M. tuberculatus and 26 samples of P. lineata were obtained, and positive reactions were observed in 29%, 48% and 8% respectively. IN conclusion, the results confirmed the WSSV presence in wild crustaceans and molluscs, which constitute a reservoirs or carrier of this agent, and a potential risk to local shrimp farming. This is the first report of WSSV in wild M. tuberculatus and P. lineata.