Avaliação dos biomarcadores antioxidantes e hematológicos em equinos marchadores suplementados e submetidos ao teste de simulação de marcha modificada

Abstract

The physical exercise is the most physiological stressful stimulus that exists, because it submits the organism to the great changes aiming at the maintenance of the muscular activity. The present study measured hematological biomarkers, digestion and oxidative stress in the blood of horses submitted to gait simulation test, and horses in maintenance and training, treated with a mixture of oils rich in omega 3 and 6. In the experiment 1, twelve Mangalarga Marchador animals were used, and the animals were submitted to the low intensity and medium duration gait simulation test (MST), 4 tests and 2 animals per day, blood samples were collected (T2), after 15 minutes of recovery (T3) and after 4 hours of TSM (T4), and were evaluated: CGV, Hb, HT, CGB, LINFO, VCM, CHCM, PLAQ, CV, RDW-SD, GPx, SOD, PPT, URE, CREAT, ACUr, CK, ALT, AST. In experiment 2: Two different groups of activity were used, one in maintenance (6 adult females) and the other in training for gait tests (10 adult horses), the groups received 100mL and 300mL, respectively, of supplementation of a mixture of oral oils for 8 weeks. Blood samples were collected in three different periods: pre-test, after 4 and 8 weeks in the morning, with fasting animals at 12 hours and at rest, and evaluated: CGV, Hb, HT, CGB, LINFO, RDW-SD, GPx, SOD, PPT, AcUr, CK. We conclude that MST causes changes in biomarkers of oxidative, protein and hematological stress in a significant way, which should be considered in this group of athletes who can participate in competitions with reduced intervals during gait tests. And that the supplementation with polyunsaturated oil and vitamin E raises the blood antioxidants in animals in maintenance and training, with different standards, but contributes to the fight against the oxidative stress characteristic of each group analyzed.