Marcadores microssatélites para o pacamã (Lophiosilurus alexandri, Steindachner, 1876) : uma espécie endêmica ameaçada de extinção no Rio São Francisco

Abstract

The pacamã (Lophiosilurus alexandri) is a species of endemic catfish São Francisco river belonging to the order Siluriformes. It is a species very appreciated in the regions of the São Francisco River by the absence of intramuscular bones and quality of their steak, also highlighting the great potential for aquaculture. The pacamã among the most endangered species and restocking programs in the various regions of this basin have been developed. The aim of this study was to develop novel microsatellite markers for pacamã that can be used for genetic studies of this species and their populations. Tissue samples were collected from adult fish caught by artisanal fishing between the reservoirs of Sobradinho and Itaparica-BA-PE. First, a genomic DNA library was performed and a set of microsatellite was obtained. After genotyping, the number of alleles ranged from two to nine, and the average heterozygosity were 0659 (He) and 0.603 (Ho), respectively. Only one marker showed deviation from HWE and presence of null alleles. The overall coefficient of inbreeding (FIS) was 0.091, suggesting the presence of homozygous, which may be due to species characteristics. Polymorphic information content is, in most cases, greater than 0.5, confirming the efficiency of these markers for genetic studies