Validação e aplicação de método para determinação de oxitetraciclina por cromatografia líquida de alta eficiência (CLAE) em camarão

Abstract

The white shrimp Litopenaeus vannamei is the species that stands out most in brazilian shrimp culture. One of the threats to the growth of this activity is the occurrence of diseases that affect the cultured animals, and hence, one of the actions taken to prevent or treat sick animals, the use of antibiotics, such as oxytetracycline (OTC). High Performance Liquid Chromatography (HPLC) with Detection Array of Diodes (DAD) was used to evaluate the OTC’s levels in shrimps cultured in tanks and treated with the drug. After the end of the treatment of shrimps with medicated feed containing OTC at 0,2; 0,4 and 0,5 mg/kg for 14 days, the elimination of antibiotic in muscle and the carapace of the animals until 22 days after medication was evaluated. It was verified through the results that the validated method intended to use and the OTC depletion was greater in carapace of animals (from 10 to 13 days) when compared to the muscle (five days). There was no statistical difference (P 0,05) between treatments used for levels of OTC in muscle and carapace. It is essential to point out the importance of quality control of drugs used in aquaculture as well as the deficient review of national and international standards about the use in the analyses of the shrimp whole, and not just the muscle, to evaluate the accordance of OTC residue in sample of crustaceans.