Caracterização da resposta inflamatória induzida por uma fração proteíca do látex de Cryptostegya grandiflora R.Br. em modelos experimentais in vivo

Abstract

The Cryptostegya grandiflora R. Br. is a member of the family os Asclepiadaceae has found is Northest Brazil. Its presents the potential of multiporposes, as an example of material-raw to industry and in the pop medicine for its antibacterial potential, stimulant and anti-inflammatory. This paper may suggest an biotecnical applying to latex of C. grandiflora as a substance to manufactor alternative medicines with anti-inflammatory porposes and/or pro-inflammatory to several pathological and fisiological conditions. The objective of this work was to evaluated the mechanism and possible mediators envolvible to the induced inflammatory response by fraction LP the latex of C. grandiflora. The model used have been: peritonitis, paw edema, air pouch e model of cutaneous wounds. Its has been used Swiss mice (38,0 ± 3,0 g) or Wistar Rats (170,0 ± 33,0). Its has been checked that the fraction LP latex induced the migration of neutrophhils to peritoneal cavity so that dose and time dependent in the peritonitis model, paw edema and air pouch. The increasing number of macrophages through pre- treatement of the animals with thiglycolate potencialized the migration of neutrophils induced by fraction LP the latex of C. grandiflora; The depletion of mast cells through the treatment with substance 4880 did not interfere in the migration of neutrophils induced by fraction LP the latex. This way, if supposes the involvement of the macrophages and not mast cells in the induced inflame response by fraction LP the latex. The pro-inflammatory activity in the model of peritonitis has been inhibited through the pre-treatment of the animals with tthalidomide, dexamethasone, indomethacine and celecoxib. The fraction LP latex a applied by endovenous may reduced the migration of neutrophils by carrageenan in rats and increased the presence of inflammatory signals oedema, hyperemia, exudative characteristics and crust in cutaneous wounds of mice and was not capable to stimulate the process of repaiting of these cutaneous injuries.