Aspectos morfológicos, morfométricos e ultra-estruturais do baço de ratos após o clampeamento total do pedículo hepático

Abstract

One of the complications of hepatic pedicle total clamping is the splanchnic congestion and consequently a splenic congestion. The aim of this study was to evaluate the macro and microscopic alterations that occurred in the spleen during an ischemia produced by the hepatic pedicle total clamping. Fourthy male rats were divided in four groups of ten animals each. The control group (C) was not submitted to ischemia and the experimental groups (E1, E2 and E3) were submitted to the clamping during 10, 20 and 30 minutes, respectively. After these periods, spleen fragments were collected and histological analyzed by the eosin-hematoxilin and ferric ferrocyanide staining technique. Based on the results it is possible to conclude that 10 minutes of hepatic pedicle total clamping is sufficient to show signs of splenic congestion and 20 and 30 minutes promote intense red bood cels digestion by the macrophages with the presence of iron granules (hemosiderin) at the splenic parenchyma.