Pesquisa de anticorpos anti-Leptospira spp. e anti-Brucella abortus em bovinos abatidos em matadouro público no estado de Pernambuco

Abstract

Given the economic impact with health public caused Leptospira spp and Brucella abortus, and absence prevalence of Leptospirosis and brucellosis in beef cattle in Pernambuco State, the purpose this work was to estimate the prevalence of anti-Leptospira spp. and anti- Brucella abortus in cattle slaughtered in abattoir public in the Pernambuco State. 412 animal’s blood samples were collected after section of the great vessels of the neck during bleeding, and serum obtained in Bacteriology Laboratory University of Course of Medicine Veterinary of Universidade Federal Rural de Pernambuco. These were processed at Laboratório Nacional Agropecuário de Pernambuco, according to official methodology. For detect antibodies anti-Leptospira spp. was used the Microscopic Agglutination Test (SAM). The samples were diluted 1:50 and screening with 27 Leptospira spp. Were positive serum with titer equal or more than 100, Leptospira spp. agglutination equal or more than 50%. Screening of Brucella abortus was performed with Buffered Acidified Antigen (AAT) in order to identify possible reagent samples to test Complement Fixation Complement (TFC). The statistical analysis of the samples was performed by descriptive analysis of them. For Leptospira spp., of the 412 samples tested, 55 were positive (MAT> 1:100), resulting in a seroprevalence of 13.3% (95% CI = 10.1% and 16.6%). Positive animals were from 20 of the 24 counties of origin. Of the 24 serovars studied, 16 were detected: Shermani (25.5%), Wolffi (14.5%), Hebdomadis (10.9%), Grippotyphosa (9.1%), Canicola (7.3%), saxkoebing ( 5.5%), Bratislava (5.5%), Copenhagen (3.6%), Hardjo (3.6%), djasiman (3.6%),Icterohaemorrhagiae RGA (1.8), Icterohaemorrhagiae Icteria No. 1 (1.8), Serjoe (1.8%), Panama (1.8%), Pyrogenes (1.8%) and Patoc (1.8%). The antibodies anti-Brucella abortus were not detected, giving a prevalence of 0%. Although there are not cattle with brucellosis, the animals evaluated offer risk for health public, because the people have chance to acquire Leptospirosis through them.