Uso de selênio e vitamina e na proteção contra danos oxidativos sistêmicos em caprinos induzidos à insulação escrotal

Abstract

To evaluate the effect of supplementation with selenium and vitamin E in the diet of goats submitted to scrotal insulation (IE) on the profile of biochemical markers of systemic oxidative metabolism, we used 12 animals which were randomly divided into two groups (G1 = no supplementation G2 = supplemented with selenium and vitamin E). The supplementation started 60 days before the induction of insulation (IE). At the end of the induction period of 30 days, began to IE with placement of plastic bags in the testes for 18 days. Supplementing with Selenium and Vitamin E for the animals of G2 was maintained for 42 days after the end of IE, corresponding to the post-insulation (PIE). Blood samples were obtained by jugular venipuncture for analysis of reduced glutathione (GSH), thiobarbituric acid reactive substance (TBARS-MDA) and determining the ferric reducing ability of plasma (FRAP). There was a significant decrease in GSH during the IE and then in the PIE, there was a gradual increase of this variable. There was no statistical difference between groups. The determination of TBARS, no significant differences between treatments and times of collection. We recorded significant differences in FRAP for collection times. As observed in the lower mean 12 and 18 days of IE. There were no differences between groups. The use of supplemental dietary selenium and vitamin E did not reduce the concentration of free radicals formed in a position to scrotal insulation, however the model of induction of testicular heat stress was effective in promoting systemic oxidative stress. The FRAP method can be recommended as a biochemical marker of oxidative metabolism in goats with insulation.