Eletrólitos e biomarcadores sanguíneos em equinos submetidos ao teste padrão de simulação de marcha

Abstract

The standard four-beat gaited simulation test (TSM) had been used to evaluate four-beat gaited horses´s fitness levels. The objectives of this research were evaluated adaptation of blood electrolytes and biomarkers in equine submitted to TSM. It was used 16 horses (Campolina n=8 and Mangalarga-Marchador n=8), males and females, Age ~6,6 years-old. Blood samples were collected in: fasting (T0), immediately after the warm-up (T1), after 15 (T3) and 30 (T4) min of “marcha” phase and 15 min after recovery period (T5). It was collected: heat rate (FC), [Glucose], [Lactate], [TPP], Hematocrit, [Ca2+], [P+], [Cl-], [K+], [Na+], [Mg2+], [Triglycerides], [Total Cholesterol Total], [Glutamine] e [Glutamate]. Also it was measured horses' speed and distance. Results were analysed by one- and two-way ANOVA and post hoc Holm-Sidak test, with P<0.05. Horses did TSM at ~3.03 m/s, and fit horses had higher [Glucose] and [Lactate] when compared with unfit group (P<0.05). In addition, [TPP] and Hematocrit were higher in T4. [Ca2+] decreased and [P+] and [K+] elevated during the TSM (P<0.05). [Triglyceride] changed significantly but not [T-Cholesterol]. Finally, HR changes but were inside the higher limit to HR for aerobic exercise. In conclusion, "marcha" or four-beat gait is a typical aerobic exercise and produce metabolic adaptation to support horse’s energetic metabolism and electrolyte concentration in the blood.