Avaliação toxicológica e anti-tumoral de uma naftoquinona sintética em tumores de Ehrlich (estudo in vivo)

Abstract

Quinones are substances of natural occurrence, with antibacterial, antifungal and antineoplastic action, between others. The 2-N-glicine-1, 4-naftoquinone, structurally related with lapachol was evaluated with respect to toxicity and antitumor activity. To toxicological analyses were used mice, Mus musculus, variety, albinos Swiss, which received by orogastric way the 2-N-glicine-1,4-naftoquinone in the 120, 240, 1000 e 4000μg/Kg doses. The lethal doses were higher than 4000μg/Kg. The dosages of 1000 e 4000μg/Kg caused cytotoxics lesions on heart, liver and kidneys. The dose of 120μg/Kg produced discrete lesion and compartmental alteration when compared with other dosages that were utilized. In antitumor evaluation were used mice, Mus musculus, variety albino Swiss to test the effect of the referred substance, in the dosages of 40, 60, 80 e 100μg/Kg, under Ehrlich tumor implanted in subcutaneous tissue of right auxiliary region, from tumor cell maintained under ascitic form. Treatments were begun on 3th day before cell tumor implantation and daily biometric evaluation were made during treatment. Seven days before was realized euthanasia of the animals and was colleted blood to leukocyte count and hematocrit. Yet, histopathological evaluations were realized about the tumor and organs how: lung, liver, kidney, spleen and heart. Microsteatosis and hepatic coagulate necrosis, Bowman`s capsule thickening and renal tubule necrosis were more intensified with higher dose. The group that showed better rate of the tumor inhibition was the one treated with 40μg/Kg. Considering the last day of treatment, the better rate of inhibition was obtained by the group treated with 100μg/Kg followed by the groups that received 80 e 40μg/Kg, respectively. The drug evaluated did not have anti-cancer effect dose dependent, however were verified toxic reactions