Efeito antimicrobiano e antiaderente in vitro de plantas da caatinga frente a micro-organismos patogênicos de interesse na área de alimentos

Abstract

This study aimed to evaluate the antimicrobial effect of ethanol extract of Hymenaea martiana Hayne and Amburana cearensis A. C. Smith, against Staphylococcus aureus, Salmonella spp. and Escherichia coli. isolated from samples of milk and cheese from farms in semiarid Pernambucano and Baiano, beyond their ATCC strains, and evaluate the antiadherent effect of Hymenaea martiana Hayne against strains of S. aureus. In the evaluation of antimicrobial activity, or the determination of minimum bactericidal concentration, was used microtiter plates containing Mueller-Hinton broth and after incubation of the material with the use of a replicator, aliquots were plated on petri dishes containing Mueller-Hinton agar, followed by further incubation of. It was observed that A. cearensis inhibited 80% of the samples S. aureus, 4,2% of Salmonella spp., but had no effect in samples of E. coli. H. martiana inhibited 100% of the samples of S. aureus, 33% of Salmonella spp. and also had no effect on isolated E. coli. Regarding the activity nonstick, by obtaining the absorbance in microplate reader held in the 595nm, it was observed that the extract of H. martiana showed 60% reduction in biofilm consolidated, and all concentrations exhibited inhibition in biofilm formation. It was concluded that the ethanol extracts of H. martiana Hayne and A. cearensis A. C. Smith showed antimicrobial activity against S. aureus and Salmonella spp., but not on E. coli and the extract of H. martiana Hayne showed antiadherent activity against strains of S. aureus. Therefore, it is suggested that the natural antimicrobial and antiadherent can offer advantages to the food area, favoring the hygienic quality and shelf life of products.