Avaliação da reação em cadeia de polimerase (PCR) e elisa indireto como método de diagnóstico da Burkholderia mallei (Mormo)

Abstract

Glanders is a highly contagious disease caused by Burkholderia mallei solipeds, a Gram - negative bacterium, not motility and aerobic coccobacillus, primarily infecting horses, donkeys and mules, though humans are considered accidental hosts . The Burkholderia mallei is listed in the list of the World Organisation for Animal Health (OIE) as an important public health disease, and due to its high potential for infection is referred to as a bioterrorism agent. According to the OIE comprises the serological diagnosis, allergy testing and bacterial isolation, and complement fixation, the official test to be performed for trade of animals. This method of diagnosis is recommended in Brazil by Normative Instruction Nº 24 Ministry of Agriculture, Livestock and Food Supply by its high sensitivity and specificity. Serological qPCR showed 76 (16.7%) positivity and a sensitivity of 24.7% and a specificity of 89 % when compared to the gold standard western blotting used. The results show that the use of the qPCR may be used as a complementary technique to other methods for rapid and accurate identification of Burkholderia mallei. About statistical results showed that the ELISAi showed the highest sensitivity (35.5%) and specificity (97.6 %) when compared to western blotting. The results show the importance of preparation and use of antigens that are produced with local strains, resulting in higher sensitivity and specificity of the test.