Ocorrência do herpesvírus bovino 5 em bovinos procedentes de matadouros do Sertão do Araripe - PE

Abstract

The knowledge of diseases that affect cattle is extremely important, the neurological manifestation of disease are frequent and caused by various agents , the Bovine herpesvirus type 5 ( BoHV - 5 ) is a virus that causes neuropathy in cattle , latency in the central nervous system ( SNC) and clinical manifestation in periods of stress. The object of the study was to research the DNA presence of Herpesvirus Type 5 in cattle through the Real Time Polymerase Chain Reaction (qPCR) based upon the utilization of two protocols and analyze the histopathological findings in samples of the cattle central nervous system. Forty-eight (48) cattle samples of the central nervous system samples were obtained comprised of brain, cerebellum, brain stem and spinal cord derived from three slaughterhouses located in the cities of Ouricuri, Bodocó and Exú, Araripe Wilderness in the state of Pernambuco. Under molecular analysis the efficiency of the protocols was compared using the following primers. With the primers used in protocol 1 there was obtained 29.16% (14/48) of the positive samples, however, the utilized primers in protocol 2 resulted in well defined peaks with regard to positivity of the positive controls and all of the samples were negative toward the qPCR. Based on histopathology analysis of central nervous system samples from Ouricuri-PE the following lesions became evident: lymphocytic infiltrate 90% (18/20), gliosis 80% (16/20), neuronophagia 80% (16/20), edema 70% (14/20), macrophages 65% (13/20), congestion 65% (13/20), demyelination 45% (9/20), bleeding 35% (7/20), necrosis 15% (3/20), gitter cell 5% (1/20), spongiosis 5% (1/20) and perivascular cuff 5% (1/20). As a result of the diversity of pathogens and similarity of neurological diseases in cattle the use of primers that present few crossed reactions (dimers) is fundamental for the efficiency and success of the qPCR against BoHV – 5.