Análise do polimorfismo no gene da Leptina em búfalas da raça murrah nos estados de Pernambuco e Alagoas e na relação com a produção leiteira

Abstract

Currently, there have been search for molecular markers in animal selection programs which could help in the classic selection, anticipating the evaluations of the animals and optimizing the genetic selection system focused on production. The hormone leptin and its receptors are mainly expressed in adipocytes and related to the control of food intake, energy use and indicator of nutritional conditions of the body that influence the actions of other physiological systems, such as milk production. Thus the present study aims to investigate the existence of LEP 1620 polymorphism (A / C) in the gene encoding the hormone leptin in buffaloes and their possible association with milk production in the Northeast region of Brazil. DNA was extracted from blood samples collected from the tails of animals. Blood samples were collected from 139 buffaloes from two farms in the Pernambuco and Alagoas. After DNA extraction, the samples were genotyped by PCR-RFLP technique. The AA, AG, GG were found. Since the AG genotype was associated with a higher milk production for the farm A when compared to farm Pernambuco. However, the genotypes presented in this study were not associated with variables for days in milk and average daily production. However, other leptin gene polymorphisms can be studied with a view to search for a marker, since there are few studies associated with this type buffalo.