Produção de biossurfactantes por leveduras isoladas do pólen apícola de Melipona seminigra merrilae da região do baixo Amazonas - AM

Abstract

Biosurfactant production was the selection factor between 20 yeasts isolated from bee pollen of Melipona seminigra merrilae of the lower Amazonas - AM. The production of biosurfactant by yeast 9II using waste as a source of carbon and nitrogen was optimized using statistical design Plackett-Burman. The metabolic liquid cell-free presented surface tension 28,30 mN/m, yield of 5,09 g/L of crude biosurfactant. The growth kinetics and production of the biosurfactant with the best production condition studied demonstrated a correlation between the growth of yeast with the biosurfactant production. The liquid metabolic presented emulsification of 100% for virgin engine oils and residual oil engine. The biosurfactant was stable before a pH range (2-12), temperature (4-120 °C) and NaCl concentrations (2-12%, w/v) and no toxicity compared to Lactuca sativa L., Brassica oleracea L. and Coriandrum sativum L. seeds and Artemia salina shrimp.