Avaliação do efeito esterilizante de solução à base de gluconato de zinco em testículos de ratos wistar em associação com anti-inflamatórios e antiálgico

Abstract

Chemical sterilization is an efficient, inexpensive and suitable for sterilization programs on a large scale domestic animals. It is believed that the injection of intratesticular zinc-based solution promotes an inflammatory reaction, and this reaction may affect spermatogenesis, taking the animal sterility. By virtue of this inflammatory reaction cause discomfort to animals, it is recommended the use of analgesics and anti-inflammatories to minimize undesirable side effects. However, the use of these drugs may inhibit the desired sterilizing agent. Therefore, the present study aimed to investigate whether the administration of anti-inflammatory steroidal, non-steroidal inhibit the effect of sterilizing solution based zinc gluconate on testicular function of rats. Therefore, 72 animals at 90 days of age were kept in vivarium conditions at Department of Morphology and Physiology, Federal Rural University of Pernambuco. These animals were divided into six groups: control / saline (G1), control / DMSO + dipyrone (G2), dipyrone (G3); Celecoxib + dipyrone (G4), dipyrone, meloxicam + + (G5); dipyrone + dexamethasone + (G6). The animals were anesthetized and injected intratesticular solution based on zinc gluconate (Infertile®). Treatment with anti-inflammatories and anti pain were conducted using a single dose per day for 7 consecutive days. After 7, 15 and 30 days the animals were heparinized, anesthetized and perfused testis and epididymis were collected for qualitative analysis of the lesions and blood plasma for testosterone measurement using the method of enzyme linked immunosorbent assay (ELISA). Regarding the histopathological lesions, the most frequent in the testes were the presence of inflammation, tubular necrosis, dystrophic calcification, congestion, edema and subcapsular tubular degeneration and atrophy of the epididymis duct, collagen deposition, and neovascularization and inflammatory infiltrate. As for the animals that underwent intratesticular injection of zinc gluconate, we found an average reduction of 74, 66 and 53% of testosterone levels in the respective experimental periods 7, 15 and 30 days. In this sense, found that intratesticular injection of Infertile® was effective on sterilization even after use of anti-inflammatory therapy. Thus anti-inflammatory steroidal and non-steroidal administered in this experiment for 7 days post-testicular infiltration can be used in chemical sterilization protocol to reduce nociceptive effects in animals infiltrate. However, the anti-inflammatory effect of dexamethasone interfered in action desired Infertile® in the first 15 days. Associations meloxicam / dipyrone and celecoxib / dipyrone may be therapy and anti-inflammatory analgesic of choice among the studied chemical sterilization protocols.