Lectinas de Cratylia mollis (Cramoll-1,4) : estudo de uma formulação em gel irradiado e seu efeito no processo de reparação tecidual in vivo

Abstract

Lectins Cratylia mollis (Cramoll-1,4) have been structurally characterized and their biological and pharmacological effects remain under investigation, since its use as a biopharmaceutical requires further elaboration. The objective of this study was to propose and characterize a gel formulation radiated Cramoll-1,4, and the preservation of properties of lectins in vitro, under the influence of gamma radiation and its activity in tissue repair processes in vivo (skin and bone). Initially, we made the extraction and purification of Cramoll-1,4, was developed a gel formulation using Carbopol as a vehicle, which was irradiated with 60Co gamma rays at a dose of 7,549 kGy h-1. To evaluate the biodistribution, Cramoll-1,4 was incubated with Tc-99 and to characterize the marking was made thin-layer chromatography. Then the gel was used in protocols for tissue repair in vivo, specifically bone tissue and epithelial. The proposed formulation at a concentration of 300 μg retained the hemagglutinating activity in vitro, suggesting a role of "protection", making the inactivation of Cramoll-1,4 radiation. The biodistribution of this gel was evaluated from the labeling with technetium showed that this lectin was captured by the intestine, metabolized by the liver and excreted by the kidneys and does not cross the blood brain barrier. The application of the topical gel irradiated Cramoll-1,4 at a concentration of 300 μg in bone defects was observed that the formulation, promoted an immediate boost in soft tissue, making it difficult, however, direct contact between the formulation and bone tissue. Thus, we observed the formation of fibrous tissue and not the expected new bone formation. In parallel, regular topical application of gel irradiated Cramoll-1,4 at a concentration of 100 μg for the treatment of second-degree skin burns, experimentally induced, sped up the process of granulation, reepithelialization and wound contraction.