Caracterização do ciclo de vitelogênese da lagosta pintada (Panulirus echinatus Smith, 1869), do litoral sul de Pernambuco

Abstract

This study aimed to characterize histologically the cycle of vitellogenesis of painted lobster Panulirus echinatus from Southern Coast of Pernambuco, in different stages of maturation. 173 females animals were collected from for a period of 12 months between the months of December 2007 to November 2008, an average of 15 animals per month, Tamandaré Beach, South Coast of Pernambuco State. Data collection was performed using gillnets and the samples were taken at random. After being captured the animals were euthanized by heat shock, ovaries and hepatopancreas were collected and individually weighed, fixed in 10% formaldehyde in water from the site of capture for paraffin and the 4% buffered glutaraldehyde for inclusion in historesine. The blocks were cuted and stained with hematoxylin-eosin, Gomori's trichrome, hematoxylin / phloxine, PAS + hematoxylin, Control, PAS, PAS + Alcian Blue pH 2.5 and Sudan Black. Histological analysis revealed four maturation stages: development (stage II) Pre-maturity (stage III), maturation (stage IV) and post-spawning (stage V) and three-stage oocytes, no vitellogenic, vitellogenic primary phase, and secondary vitellogenesis. In mature oocytes the cytoplasm is PAS positive and presence of lipid droplets positive for Sudan. Gonadossomatic and hepatossomatic indexes showed an inverse correlation in the different stages of maturation indicating a possibility of transfer of yolk from hepatopancreas to the ovaries during the reproductive period.