Avaliação da castração química com papaína associada ao acído lático em ratos wistar

Abstract

This study aimed to evaluate and chemical castration with papain associated with lactic acid in adult Wistar rats. Twenty-five male Wistar rats at 90 days of age were divided into 5 groups. The animals of the groups G1, G2, G3, G4 received 0.1 mL of papain solution and lactic acid intratesticularly and they were euthanized respectively 24h, 48h, 72h and 96h after treatment, and the animals of the control group "CG" received 0.1ml of saline and they were euthanized 24h after treatment. Fragments of testis, epididymis, kidney, and liver were collected for histopathological evaluation and blood for analysis of testosterone. The daily monitoring of the animals showed no behavioral impairment, with physiological acts within the normal range, such as eating, defecation and urination, as well as socializing with the group. kidney and liver changes were observed in the histopathological study of these organs, suggesting that only a local effect solution. Already in the testis and epididymis changes were characterized by inflammatory infiltrate, vacuolation and necrosis of germ cells. On testosterone levels G1 and G2 had values below 1 ng / ml, which technique was effective in reducing testosterone levels within 48 hours. Given the histopathological findings in the testis and epididymis, papain and lactic acid solution did not affect the kidney and liver and did not affect the behavior of animals. The solution was shown to reduce the activity of Leydig cells in the first 48 hours. But further studies are needed on the long-term effects and the application of higher concentrations of this solution in rats and the development of new projects that could cover all species.