Potencial antimicrobiano de Byrsonima sericea DC. sobre patógenos causadores de mastite caprina

Abstract

Mastitis is an infectious disease characterized by an inflammation of the mammary gland, caused mainly by infections resulting mostly from the bacteria Staphylococcus spp. and Streptococcus spp. These microorganisms are becoming increasingly resistant to current antimicrobial drugs, making it necessary to develop new compounds capable of inhibiting them. Plants are natural sources that have antimicrobial potential relevant to research, as is the case of Byrsonima species, popularly known as muricis, with applications in folk medicine. The objective of this work is to analyze the antimicrobial potential of extracts and fractions of leaves and branches of Byrsonima sericea DC against Streptococcus agalactiae and species of Staphylococcus that cause mastitis. Ethanol extracts were produced from leaves and branches, and from these extracts the hexane, chloroform, ethyl acetate and aqueous fractions were produced. The crude extracts and fractions were subjected to antimicrobial tests by the agar diffusion method and those that showed activity were subjected to the antimicrobial test by microdilution. The results showed that the crude extracts and the aqueous and ethyl acetate fractions were active against all the microbial species tested, by the agar diffusion test, with halos from 11 to 23 mm for the ethanolic extracts of the leaves and branches, in the concentrations of 25, 50 and 100mg / mL and from 7.6 to 13.6 mm for fractions in concentrations of 5 to 25mg / mL. In the microdilution test, it was not possible to determine the minimum inhibitory concentration (MIC), but it was still found antimicrobial activity against most of the bacteria tested by the extracts and fractions, with the exception of Staphylococcus aureus ATCC 25923 only. ethyl acetate, with 95% and aqueous, with 100% inhibition in the concentration of 0.195mg / mL of the leaves on Staphylococcus chromogenes. Given the need to find new compounds that act on pathogens that demonstrate resistance to conventional drugs, and the plant species having demonstrated antimicrobial action makes it of interest for progressive researches.