Estudo hematológico e proteinograma sanguíneo e do colostro de ovelhas suplementadas com propileno glicol e com cobalto associado à vitamina B12 e de suas respectivas crias

Abstract

This work aimed to evaluate the influence of propylene glycol and cobalt associated with vitamin B12 on the hemogram and blood proteinogram and colostrums of Santa Inês ewes in the period of transition and their offspring. The study was conducted in the Clínica de Bovinos experiment barn for small ruminants, Campus Garanhuns – UFRPE, using 18 pregnant ewes and their offspring (n=23). The ewes, after clinical examination, were divided randomly, 30 days before lambing, in three groups, Group 1 (G1/n=6), supplemented with propylene glycol (30mL oral way daily), Group 2 (G2/n=6) supplemented with cobalt (1mg cobalt chloride 1%, oral way daily) and vitamin B12 (2mg intramuscular way weekly) and the Group 3 (G3/n=6) control group. Supplementation of ewes was carried out for 30 days before the expected delivery date until the time of lambing. It was established for hemogram and the electrophoretic profile of blood serum of ewes eight experimental moments, 30 days before lambing, a week before lambing (antepartum), at the time of lambing, 24hPP, 72hPP, 05dsPP, 15 dsPP and 30dsPP and to assess the proteinogram of colostrum a single experimental time was established soon after lambing. For the lambs, it was established 11 experimental moments, before suckle colostrum (M0h), 6h, 12h, 24h, 48h, 72h, 7ds, 15ds, 30ds, 60ds and 90ds of life, except for the variables of the hemogram where no collection was made 6h and 12h of life. In sheep, supplementation with cobalt associated with vitamin B12 resulted in the elevation of the erythrocyte variables in the transition period, as well as the administration of both supplements in higher serum concentrations of immunoglobulin G. Among the acute phase proteins, the α1-acid glycoprotein was the most sensitive among the others identified in blood serum rising as a result of lambing. The administration of the supplements did not influence the concentration of the eight proteins identified in colostrum (IgA, lactoferrin, albumin, IgG heavy chain (IgGCP), β-casein, IgG light chain (IgGCL), β-lactoglobulin and α- lactalbumin) by electrophoresis in polyacrylamide gel. In the hematological response it was found influence of cobalt administration associated with vitamin B12 in late pregnancy on the increase in erythrocyte parameters of lambs, and the belated reversal of the neutrophil ratio: lymphocyte. The administration of the supplements did not affect the transfer of passive immunity considered moderate to good for the lambs, as well as the dynamics of the proteinogram, where the latter was influenced by age factor, particularly by higher protein ceruloplasmin, α1 - acid glycoprotein , α1 - antitrypsin and transferrin in the first two weeks of life.