Avaliação da reação em cadeia da polimerase em tempo real na detecção do Corynebacterium pseudotuberculosis em caprinos

Abstract

The caseous lymphadenitis (CL), infectious disease characterized by the formation of lesions abscedantes, especially in lymph nodes, stands out as a great economic importance because it is one of the main causes of condemnation of carcasses of small ruminants and cause severe depreciation of leather these animals. This study aimed to evaluate the polymerase chain reaction (PCR) in real time for detection of Corynebacterium pseudotuberculosis in goats. 643 goats were evaluated, of which 25 animals with lesions abscedantes surface underwent harvest of abscess contents, for the identification of C. pseudotuberculosis through bacterial isolation and real time PCR. Additionally, blood, milk and feces were also processed by real time PCR for estimating the elimination of the agent in these specimens. Of the 25 samples content abscess subjected to bacterial isolation and PCR, 72% (18/25) and 36% (9/25), respectively, were positive for C. pseudotuberculosis, there is a positive correlation between the tests. The bacterium also was detected at frequencies of 8% (2/25) 4% (1/25) and 0% (0/25) in milk, blood or feces, respectively. The real-time PCR, when applied directly to samples of abscess contents, showed no considerable reliability in the diagnosis of CL in goats, despite the results of the preliminary characterization technique in drug elimination by hematogenous and lactea routes.