Estudo dos biomarcadores cardíacos troponina I e CK-MB em cabras e ovelhas acometidas por toxemia da prenhez

Abstract

Pregnancy toxemia (TP) is a disorder characterized by metabolic, energetic, proteic, mineral and hormonal disorders, with evident clinical signs, with losses of fetuses and matrices that can exceed 80% of the affected animals. In goats and sheep with TP, ketonemia can reach up to 10 times the normal concentration in the blood, generating high amounts of free radicals that exert cytotoxic action on the membranes of the tissues, altering their permeability, reducing the action of antioxidants and causing damage to the heart. The objective of this study was to evaluate the effects of metabolic disorder caused by PT in cardiac muscle function, using specific cardiac biomarkers such as cardiac troponin I (cTnI), creatine kinase myocardial band (CK-MB) in goats and sheep affected by the disease. Blood samples were collected from goats (44 samples) and sheep (55 samples) diagnosed with TP. In serum, troponin I, CK-MB, cortisol, insulin, non-esterified fatty acids (NEFA), beta-hydroxybutyrate (β-OH), frutosamine, cholesterol, triglycerides, total proteins, albumin, globulin, urea, creatinine , aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), creatine kinase (CK). Plasma glucose determination was performed. The obtained data were analyzed and compared with groups of animals without TP. Statistical analysis was used to analyze variance (ANOVA) and Student's t-test, with a 5% significance level (p <0.05%), using the MINITAB® 18 computer program. cTnI in goats and sheep that had TP and died when compared to the TP group that had clinical discharge, and differed from the group of animals without TP. The serum concentration of CK-MB in the animals affected with TP that died was higher in relation to the control group. Changes in the energy profile were also observed in the concentrations of NEFA and β-OH, in the enzymatic activity of CK, AST and GGT, and in hormonal indicators such as cortisol and insulin. The use of these biomarkers in the clinical routine can be a good tool in the application of diagnosis and prognostic evaluation in this type of disease, enabling a better therapeutic intervention and reducing the losses of animals and economic losses.