Desenvolvimento de adsorvedores de DNA/corantes à base de fibras poliméricas eletrofiadas

Abstract

Electrospun fibers are potential candidates for use as ionic dyes adsorbents, as well as for the use in DNA extraction. With the aim to obtain low-cost fibers, the electrospinning process stands out as a simple method applied in the production of nanofibers due to its high surface/volume ratio. This work explores the influence of processing variables in the optimization of the production of electrospun fibers prepared using Eudragit® L100 and its application in the of dyes, and the application of these Eudragit® E100 with Polyethylene Oxide (PEO) fibers electrospun in the process of DNA extraction. The studies involving the Eudragit® L100 electrospun fibers have shown that corresponding fiber adsorption (measured as a function of dye concentration, pH and contact time) capacity is directly related to its diameter. The adsorption fits the Lagmuir isotherm and the kinetics data are better described by the pseudo-second order model, confirming that the physical sorption prevails, with the homogeneous distribution of active sites in fibers. The application of Eudragit® E100/PEO electrospun fibers for the fast retrieval of the DNA extraction is a very fast and efficient process without degrading the genetic material in the amplification process. The results of the adsorption test show that the sorption process is physical and with a homogeneous distribution of free active sites. Thus, it is possible to associate that the mechanism of adsorption of dye and DNA is based on the electrostatic interaction between the adsorbent and the adsorbate. As a result, Eudragit® L100 fibers (anionic character) adsorb cationic dyes and Eudragit® E100/PEO fibers adsorption genetic material. Comparison with the dye adsorption capacity of experimental systems in the literature shows that Eudragit® L100 (with adsorption capacity of 135.37 mg.g-1) and Eudragit® E100/PEO (with adsorption capacity of 44,31 mg.g-1) represent new promising and efficient systems for the removal of anionic dyes and identification of the presence of genetic materials.