Efeitos tóxicos de compostos de vanádio utilizados no tratamento do diabetes sobre os parâmetros biológicos de embriões e adultos de zebrafish (Danio rerio)

Abstract

The toxic effects of sodium metavanadate (MV), vanadium pentoxide (PV) and oxovanadium sulfate (SV) on embryos and adults of zebrafish (D. rerio) were evaluated. Embryos were exposed to concentrations of 10-1000 μg/mL for evaluation of 96-h LC50 and their teratogenic effects. Adults were exposed to 20 μg/mL to evaluate behavioral changes related to vanadium compounds exposure and daily survival. Embryos showed a 96-h LC50 of 74.14 for PV and 100% mortality at the concentrations of 400-1000 μg/mL of the three compounds. The most significant teratogenic effects were pericardial edema and yolk sac (p < 0.05). The adults exposed to the vanadium compounds presented higher opercular beating and darkening of the gill arches. Floating and Resting behaviors in exposed adults were significant (p < 0.05), as well as the behavior of air breathing behavior. It can be concluded that in tests with the embryos the vanadium pentoxide is the most toxic compound and that pericardial edema and yolk sac were the most significant teratogenic effects and that the exposure of adults to vanadium compounds promoted an increase in the frequency of behaviors Inactive and stress response.