Identificação do M. bovis em amostras sorológicas pela PCR em rebanhos de bovinos leiteiros de Pernambuco

Abstract

The Mycobacterium bovis belongs to the Mycobacterium tuberculosis Complex and is responsible for tuberculosis in bovines and buffaloes, as well as in humans. This study aimed to carry out biomolecular assays using PCR for investigate M. bovis in serum samples of bovine subjected to the tuberculin test. A hundred samples, from seemingly healthy bovines and previously submitted to the tuberculin test, raised in six properties from different cities of the State of Pernambuco, were examined for Genomic identification of M. bovis. The samples were chosen at random by non-probability convenience and distributed in six groups. From the samples subjected to PCR, 5% (100/05) resulted positive for M. Bovis. The tuberculin test showed a positive reaction on 19% (19/100) of the animals. Was possible to conclude that the real time PCR, when applied directly in blood serum, showed low sensitivity to be used as a tool in routine diagnosis of tuberculosis in bovine.