Caracterização fenotípica de estirpes bacterianas oriundas de nódulos radiculares de Mimosa tenuiflora (Willd) Poir. E Desmanthus pernambucanus (L.) Thellung

Abstract

The cultural characterization of bacterial isolates is usually the first step in the process of getting the isolated and evaluation of biodiversity. These evaluations are important and allow for a preliminary screening of isolated by separating them into groups genera, for example. This study aimed to characterize phenotypically bacterial strains derived from root nodules of forages: Desmanthus pernambucanus and Mimosa tenuiflora grown in fertilized soil or not. Samples of soil for cultivation of plants in the screened greenhouse were collected in the cities of Arcoverde, Ibimirim and Serra Talhada, Pernambuco. The isolates were characterized as the time of growth of colonies, determine the shape and diameter, high transparency, exopolysaccharides production and coloration of the colonies, change of pH. They determined the number and biomass of nodules, production indoles and phenotypic characterization of bacterial isolates. Cultural assessment, were seen as key features: rapid growth time, flat elevation, exopolysaccharides production of low to moderate color ranging from cream to white and smooth surface. Legumes had nodulation in the three soils in the absence or presence of manure. Manuring does not increase the number of nodules of Mimosa tenuiflora plants, but increases the number of nodules Desmantus pernambucanus. The bacterial strains are capable of producing indole compounds without the addition of tryptophan.