Controle bacteriano na eclosão e enriquecimento de Artemia sp. para sua aplicação na alimentação de pós-larvas de Litopenaeus vannamei

Abstract

This study aimed to evaluate the antibacterial effects of different supplements on Artemia sp. hatching and enrichment. The supplements were added to the water used for Artemia hatching of capsulated and decapsulated cysts and for Artemia enrichment water. The experiment consisted in the addition of the diatom Chaetoceros calcitrans, a commercial probiotic (Bacillus spp.), antimicrobial Florfenicol to the hatching water and control without supplements. The enrichment experiment was performed by the application of C. calcitrans, probiotic and commercial emulsion DHA / EPA rich to enrichment water and control constituted by newly hatched nauplii. The enriched Artemia were offered for the PL7 to PL19 of Litopenaeus vannamei stages. The Vibrio spp. load of hatching water and newly hatched nauplii were quantified at the end of hatching. The Vibrio spp. quantification of postlarvae, enriched Artemia and water of enrichment and postlarvae rearing was also performed. The Vibrio presumptive colonies isolated from newly hatched nauplii were identified. Furthermore, Vibrio spp. present in nauplii subjected to freezing and Bacillus spp. colonies of Artemia and postlarvae of Probiotic treatment were quantified. Assessing the overall results of the study, the decapsulation process did not shown to be effective in reducing the Vibrio spp load of nauplii and water in all treatments. The C. calcitrans addition in Artemia hatching water has proven to be an effective alternative to antibiotic use. The probiotic use must also be considered to control Vibrio spp. load in Artemia nauplii. However, the supplements use to Artemia enrichment process may promote a bacterial increase and other procedures for its control must be evaluated.