Detecção de Ureaplasma spp. e Mycoplasma agalactiae pela técnica da Reação em Cadeia da Polimerase (PCR) em sêmen de reprodutores ovinos

Abstract

The objective of this study was to detect the DNA of Ureaplasma spp. and Mycoplasma agalactiae in sheep semen by the technique of Polymerase Chain Reaction (PCR). Were analyzed 240 samples, 120 frozen semen obtained from central artificial insemination (AI) and 120 fresh semen of breeding sheep from the states of Alagoas, Ceará, Paraíba, Pernambuco and Rio Grande do Norte collected during the Northeastern Exposure animals in Recife. After collection of the samples were carried out DNA extraction and detection of genomic DNA of the agents studied. For the first time in Brazil, was detected the presence of agents Ureaplasma spp. and Mycoplasma agalactiae in samples of frozen semen of sheep. Samples of frozen semen, DNA was detected Ureaplasma spp. in 2.5% (3/120) and Mycoplasma agalactiae of 4.2% (5/120). For fresh semen was detected DNA of Ureaplasma spp. in 8.3% (10/120) of the samples and 6.7% (8/120) of Mycoplasma agalactiae When evaluating the association between the type of semen and DNA detection for Ureaplasma spp. and Mycoplasma agalactiae, a significant association was observed only for Ureaplasma spp. (p = 0.046), being more common detection of this micro-organism in fresh semen samples. We also observed positive samples Ureaplasma spp. and Mycoplasma agalactiae from the states of Paraíba, Pernambuco and Rio Grande do Norte. All samples from the states of Alagoas and Ceará results were negative. Results obtained in this study, there is the presence of DNA of these microorganisms in semen of the breedings sheep. Thus, it is suggested that techniques for detecting these agents must be used in insemination centers and breeding sheep with high genetic potential to maximize the efficiency of the sheep reproduction by preventing the spread of these pathogens.