Alterações morfológicas esplênicas em ratos após o clampeamento total do pedículo hepático

Abstract

Experimental models of hepatic ischemia have been very widespread. The Pringle maneuver, i.e. clamping by vascular tongs of the artery, vein and hepatic duct, and of the portal vein is used in many of these models. These models are used for studies in liver transplants. One of the complications in this maneuver is the splanchic congestion. This study has as objective the observation of the changes that occur in the spleen due to the ischemia produced by the clamping of the hepatic peduncle. For this 36 male mice were divided into four groups with 9 animals each. The Sham (S) group was not submitted to ischemia, and the groups E1, E2 and E3 were submitted to the Pringle maneuver for 10, 20 and 30 minutes, respectively. After these periods, fragments of the spleen were taken, fixed in formaldehyde and processed for inclusion in paraffin. The slits were dyed with Haematoxylin and Eosin and with Ferric-ferrocyanide. The results showed microscopic alterations in groups E2 and E3. There was intense vascular congestion, hemosiderin granules, decrease in the white pulp (lymphatic nodules and periarterial sheaths) and intense hemocateresis around the splenic sine curves, being these changes more intense at 30 minutes. In this group there was an accentuated digestion of red blood cells with the presence of hemosiderin granules. The data obtained permit us to conclude that at 20 minutes signs of spleen congestion begin and that at 30 minutes there is decrease in splenic parenchyma and increase in concentrations of ferric pigments.