Bactérias gram positivas com potencial probiótico isoladas de camarões marinho cultivados

Abstract

Probiotics are responsible for a new idea of using preventive in aquaculture, aiming at sustainable farming, environmental balance and of its products generated. However, there are few knowledge on the subject, despite its widespread use in shrimp farming, randomly in the most of times. Because of large demand from the sector for new alternatives, to maintain animal health, reduce the chance of having disease problems that the study aimed to identify and test bacteria with potential probiotic isolated from the intestinal tract of cultured shrimp (Litopenaeus vannamei) in salt water coming from commercial farm in the state of Pernambuco. Has been collected 90 animals, 50 in rainy season and 40 in dry season. The shrimps collected during the rainy season had weight of 9.50 ± 0.52 g and length of 6.72 ± 0.84 cm, while the animals from the dry period had weight of 9.76 ± 0.24 g and a length of 6, 54 ± 0.42 cm. Were obtained 72 bacterial isolates then performed the Gram 31 were tested in vitro against dual-known pathogenic species, Vibrio parahaemolyticus (ATCC 17802) and Vibrio vulnificus (ATCC 27562). Eight isolates (28.81%) showed antibacterial activity to at least one pathogen. There was no significant difference (P <0.05) in the proportion of isolates potential probiotics obtained in different seasons, rainy and dry. Isolates that showed the best results in the antagonism test in vitro were identified as Lactobacillus paracasei, Bacillus cereus, Lactococcus lactis subsp. lactis, Lactobacillus crispatus and Lactobacillus brevis. The largest zone of inhibition observed in the antagonism test was produced by Lactobacillus crispatus opposite V. vulnificus. All bacteria tested against the pathogens studied produced inhibition zone, however it is suggested the testing of antagonism in vivo to be proven the effectiveness of probiotic bacteria for marine shrimp.