Qualidade da água utilizada em serviço de alimentação de hotéis

Abstract

Food is one of the key elements of tourist motivation and its quality is directly related to drinking water. This study aimed to assess the quality of the water used in food service hotels of the 6th Regional Municipality of Jaboatão Guararapes, PE, Brazil. 15 hotels were inspected and collected 31 water samples for physico-chemical and microbiological between June to December 2009. The hotels were grouped into large (HG) and medium (HM). To determine the Most Probable Number (MPN) of total coliform (TC), thermotolerant (Ct) Broth Lauryl Sulfate Tryptose (CLST) and Pseudomonas aeruginosa in Asparagine Broth used the technique of multiple tubes. A reassessment of the identification and confirmation of fecal coliform samples was performed by PCR. For quantification and identification of Aeromonas samples were used to direct plating method on starch agar with 20 ug / ml ampicillin and add starch 10g/1L for counting pattern (CFU / mL). All health establishments showed irregularity. The turbidity, sulfate and nitrite showed normal values, while chloride, pH and residual chlorine were out of specification. Water wells were contaminated by 25.0% (HG) for CT and Ct and 44.4% and 55.5% (HM) respectively. In reservoirs, the percentage of contamination hotels showed 75.0% (HG) and 77.7% (HM) for CT and Ct. It was observed that 66.6% of the reservoirs showed no contamination at the main entrance of the well water and public water supply. Of the 104 isolates of coliforms analyzed, 15% were species pathogenic environmental and 53.85% have been identified Buttiauxella harsh, Enterobacter aerogenes, Enterobacter gergoviae, Klebsiella ozaenae, Klebsiella planticola, terrigenous Klebsiella, Serratia liquefaciens and Serratia odor, Citrobacter amalonaticus, Citrobacter sp, Enterobacter sp, Enterobacter sakazakii, Escherichia coli, Klebsiella pneumoniae, Kluyvera ascorbata, myxofaciens Proteus and Providencia sp. The 41 isolates of Escherichia coli have been identified: Stx (-), eae (+) 3 (7.31%); Stx (-), eae (-) 21 (51.21%); eae (+) 1 ( 2.43%); Stx (+), eae (+) 1 (2.43%); StxM1M2 (-), eae (-) 10 (24.3%) PAA (-) aggR (-) 4 (9.75%) PAA (-) aggR (-) multicoli (+), eae (+) EPEC 1 (2.43%). Six (14.63%) strains of E. coli showed virulence factor, with 16.6% originating from reservoirs (HG) and 83.33% (HM). In the pit, Pseudomonas aeruginosa was detected in 25.0% (HG) and 75.0% (HM) and the reservoirs contamination was 75.0% (HG) and 77.7% (HM). , Aeromonas spp were isolated from all water samples (HG) with contamination ranging from 9.0 x 10, 1.9 x 105 wells and 2.0 x 10, 1.4 x 103 CFU / mL in the reservoirs. As for (HM) variation was 2.6 x10, 2.0 x104 CFU / mL in the well and 4.0 x101, 25 x 105 CFU / mL in the reservoir. Of (HM) that used the public water supply values were obtained from 4.0 x 103, 7.0 x 102 CFU / mL. Of the 48 isolates of Aeromonas species were 81.2% and 18.8% environmental pathogenic species have been identified A. schubertii, A. encheleia, A. eucrenophila, A. sober, A. veronii veronii, A. caviae, A. jandaei . The water of the hotel chain of Jaboatão Guararapes -6 th Region showed unsatisfactory levels of bacteriological quality of drinking water, although the water source, coming from public water supply be of good quality and free from contamination as the total and fecal coliforms. The lack of sanitation, rationing and intermittent supply of treated water, requiring the use of water reservoirs which favors the increased likelihood of water contamination.