Contagem de hemócitos associada aos parâmetros de higidez de camarões marinhos Litopenaeus vannamei (Boone, 1931)

Abstract

The blood cells normal values knowledge is one of the most valuable clinical labs technique which allows the systemic and morphologic anomaly, when identification is not cleary shown in the organism, identification. At the mammals medicine these values are great known, but on the shrimp culture there still be too much to clear up about haemolynph. Several studies have been realized aiming determines the different kinds of cells and their respective functions. However, the important information like the normal values of the hemocytes total counting (HTC) in different development phases to the Litopenaeus vannamei species and the existence of hemocytes behavior variations on males and females, as well as its relation to anatomo-histopatologic alterations, still being unknown. In face of the exposed, it was aimed to verify the hemocytes behavior on the different cultivated L. vannamei shrimp development phases, the sexual quantitative variation existence, the sodium citrate and Ethylenediaminetetraacetic acid (EDTA) efficacy as anticoagulant, beyond its relation to the detected alterations at the fresh and histopathology exams. 20 samples of haemolynph were collected weekly, being ten (five males and five females) using the sodium citrate anticoagulant and ten using the EDTA anticoagulant. The collects were realized in two aquariums (A and B), on summer and winter times at a commercial shrimp culture located in Goiana/Pernambuco estate. 0,1mL of haemolynph on a proportion of 1:1 (anticoagulant with 10%concentration:haemolynph) were collected from each shrimp to clinical pathology analyses. The hemocyties/mm3counting was realized immediately after the collect and after a five hours interval. The results were statistically analyzed using multiple linear moulds (P<0,05). The entire sample animals showed themselves healthy doesn’t presenting significant interference of the detected alterations on the fresh and histopathology exams over the HTC. The male shrimps presented bigger HTC than the females, thus the animals age interfered at the HTC, getting bigger counting on elder animals. In relation to the seasonality, the winter cultivated animals presented bigger counting than the summer cultivated. Both the sodium citrate and the ETDA preserved correctly the samples designated to HTC immediately after the collect. It was concluded that the sex, the age and the seasonality interfere directly over the HTC and the sodium citrate at 10% and the EDTA at 10% are indicated to the HTC immediately after the collect.