Pesquisa de anti-corpos IgG contra Toxoplasma gondii (Nicolle & Manceaux, 1908) em leite de inquérito soro-epidemiológico da infecção em cabras (Capra hircus, Linneus, 1758) lactantes de criações de subsistência do Município do Jaboatão dos Guararapes - PE – Brasil

Abstract

Despite its immunological properties for the newborn, milk is also considered a potential source of transmission of pathogens, and some of them cause zoonoses. The non-pasteurized goat milk has been implicated as an important source of infection of T. Gondii in children of rural areas which are feeding habit of eating raw milk. .Serological studies carried out in the Zona da Mata of the state of Pernambuco - Brazil shown that Toxoplasma gondii is widely disseminated in the goat herd in the region. The objective of this work was to find the presence of IgG immunoglobulin against T. Gondii in milk and to conduct an epidemiological investigation of the infection in lactating goats from creations of subsistence of the municipality of Jaboatão dos Guararapes – State of Pernambuco - Brazil. Samples of blood and milk were collected of 68 lactanting goats of various ages and races, in five rural properties of the said council. An investigative questionnaire was applied to each property. Samples were processed and tested by indirect immunofluorescence test for search of IgG, adopting the cut-off of 1:16 for both, followed by dilutions in factor 2 up to the dilution of1:516 for the serum and 1:64 for milk, for samples seropositive in the previous dilution. It was obtained seropositivity of 88.23%, 77.94%, 76.47%, 70.58%, 58.82% and 50% for dilutions of 1:16, 1:32, 1:64, 1:128, 1:256 and 1:512 respectively. Positivity in the samples of milk was 39.70%, 29.41% and 7.35% for dilutions of 1:16, 1:32, 1:64, respectively. Concluding that there is a statistical correlation between the result of association found in milk and in serum and in milk goats that are capable of eliminating the class IgG antibodies through milk at different times of lactation.