http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4342 2025-09-17T03:10:32Z 2025-09-17T03:10:32Z CASTRO, Valéria Milena Dantas de http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9696 2024-07-24T21:14:38Z 2024-02-16T00:00:00Z

Título: Efeitos da oxigenoterapia hiperbárica no duodeno de ratos com diabetes induzida por estreptozotocina Autor: CASTRO, Valéria Milena Dantas de Primeiro orientador: EVÊNCIO NETO, Joaquim Abstract: Diabetes Mellitus (DM) is a metabolic disorder that results in persistent hyperglycemia resulting from a lack of insulin or failures in its action. This disorder is generally accompanied by changes in cellular redox state signaling pathways that result in inflammatory responses and subsequently in morphological damage to tissues and organs, complications associated with diabetes. Given the damage that DM causes to patients, many studies have been testing alternative therapies that can reduce these complications, such as tissue hyperoxygenation. Hyperbaric Oxygen Therapy (OHB) involves inhaling pure oxygen at high pressure. In the present study, the aim is to evaluate the effects of (OHB) on the clinical, morphological, inflammatory and oxidative parameters of the duodenum of diabetic rats. For this, 33 Wistar rats aged 60 days, weighing around 220-270g, were used. The animals were distributed into four experimental groups: control group composed of healthy animals (C), control group treated with hyperbaric oxygen therapy (C+OHB), diabetic group without treatment (D) and diabetic group treated with oxygen therapy (D+OHB). DM was induced with an intraperitoneal injection of streptozotocin 60 mg/kg. It was used inside the chamber at 2.5 absolute atmospheres (ATA) at a rate of 2 ATA/min and maintained at that pressure for 60 min. The chamber was ventilated with 100% O2 at a flow rate of 20 L/min in order to minimize CO2 accumulation for a period of 5 weeks. After euthanasia, the entire duodenum was removed, where 5 centimeters were allocated for histomorphometric, immunohistochemical and stereological analyzes and 2 centimeters for marking reactive oxygen species (ERO). It was found that OHB improved polyphagia and reversed changes in the reference volume of the duodenal lumen and absolute volume of the submucosa. It also improved the expression of Caspase-3, VEGF, SOD-1 AND GPX. From the results we conclude that hyperoxygenation in this study, despite not reducing blood glucose, had positive effects at tissue level, reducing morphological changes in volume as well as improving the expression of proteins involved in apoptosis, inflammation and oxidative stress. Instituição: Universidade Federal Rural de Pernambuco Tipo do documento: Tese

2024-02-16T00:00:00Z SILVA, Francine Maria de França http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9695 2024-07-24T20:57:24Z 2024-02-26T00:00:00Z

Título: Estudo clínico-epidemiológico e terapêutico da esporotricose felina Na Região Metropolitana do Recife Autor: SILVA, Francine Maria de França Primeiro orientador: MOTA, Rinaldo Aparecido Abstract: Sporotrichosis expansion in Brazil highlights the potential zoonotic and epizootic transmission of fungal species Sporothrix brasiliensis to poorly investigated regions, such as Northeastern Brazil. The aim of the current study is to describe clinical, epidemiological and therapeutic features of feline sporotrichosis and Sporothrix brasiliensis isolates’ susceptibility in vitro in cats from Recife Metropolitan Region (RMR). The diagnosis of cats with suspected sporotrichosis was achieved through fungal isolation in culture and Sporothrix spp. species identification was performed based on the PCR technique. Information on clinical, epidemiological and therapeutic variables, as well as guardians’ addresses for georeferencing purposes, were collected through questionnaire application. Cats meeting the inclusion criteria were subjected to five different treatment protocols. Itraconazole (ITZ), amphotericin B (AnB), terbinafine (TBF) and potassium iodide (KI) minimum inhibitory concentration (MIC) against the investigated isolates was determined. S. brasiliensis was confirmed in 100% of tested samples. The epidemiological profile highlighted higher frequency of adult (85.5%), male (61.8%), semi-domesticated (82.7%) cats who had contact with soil (92.7%) and lived in houses (81.8%). Recife City (63.6%) presented the largest number of recorded cases. Ulcer (89%), crust (77.3%) and sneezing (61%) were the most frequent clinical signs of feline sporotrichosis. The disseminated cutaneous + mucocutaneous clinical form of it (32.7%) was the prevalent one, mainly in animals’ ears (50%), nasal region (45.5%) and pelvic limbs (45.5%). Animals in the ITZ group (65.2%) recorded the highest healing frequency. On the other hand, animals treated with ITZ + KI presented the highest death frequency (56.2%). Three non-wild isolates were identified in the ITZ group, whereas six non-wild isolates were identified in the TBF group. The current study enabled assessing feline sporotrichosis behavior in a given region in Northeastern Brazil that remains poorly investigated. Moreover, the herein observed susceptibility pattern in vitro was consistent with the good therapeutic response presented by cats treated with ITZ. Instituição: Universidade Federal Rural de Pernambuco Tipo do documento: Tese

2024-02-26T00:00:00Z NASCIMENTO, Janilene de Oliveira http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9694 2024-07-24T20:12:35Z 2023-08-24T00:00:00Z

Título: Monitoramento terapêutico de cães com infecção natural por Leishmania infantum tratados com marbofloxacina combinada ao alopurinol Autor: NASCIMENTO, Janilene de Oliveira Primeiro orientador: ALVES, Leucio Câmara Abstract: The treatment of dogs infected with Leishmania infantum has been a challenge for the veterinarian, because it is an expensive and long-term therapy with limited therapeutic options. Thus, the treatment in canine leishmaniasis requires laboratory monitoring, as clinical and immunological response and parasite load of the dog. The objective of this study was to evaluate the combination of marbofloxacin and allopurinol from a clinical perspective and antibody production profile in therapeutic monitoring for CanL (Canine Leishmaniasis). Twenty-six dogs with positive parasitological diagnosis (amastigotes forms the Leishmania) were divided into two treatment groups: G1 - 15 animals treated with marbofloxacin and allopurinol, and G2 - 11 animals treated with miltefosine only. The dogs were monitored for 6 months with clinical evaluations on days D0, D30, D90, and D180, and quantitative serology was performed only at the beginning and end of treatment. Clinical parameters were classified according to severity on a scale of 0 to 3.The qPCR was based on the amplification of L. infantum kDNA (kinetoplast minicircle). In the first three months, animals in G1 and G2 showed reductions of 86.6% and 45.4%, respectively, in clinical scores. Clinical signs relapsed in 9.1% of G2. At the end of treatment, the mean reduction in clinical scores was 61.3% for G1 and 22.3% for G2. Antibody titers decreased by 93.3% with the use of marbofloxacin and allopurinol, compared to 63.6% using miltefosine alone at the end of treatment. In both groups, dogs with high clinical scores had high antibody titers (D0), and as clinical scores decreased on D180, antibody titers also significantly reduced (H = 19.2506; p = 0.0002). Prior to the start of treatment, the mean number of parasites/μL in the bone marrow was, for G1, 239,267 with a median of 13,792 parasites/μL. In G2, the mean was 965,795.1 parasites/μL with a median of 117.8 parasites/μL. Marbofloxacin with allopurinol proved to be viable for reducing parasite load. Monitoring dogs treated with marbofloxacin and allopurinol for six months allowed us to observe a significant clinical improvement, with clinical cure in 20%. In an attempt to achieve a better therapeutic response, the unprecedented proposal for the combination of marbofloxacin and allopurinol demonstrated a potential protocol for use in dogs with Lcan, mainly in Brazil, as there is only one medication licensed for this, with gastrointestinal effects present. However, new studies may demonstrate a reduction in infectivity in dogs. Instituição: Universidade Federal Rural de Pernambuco Tipo do documento: Tese

2023-08-24T00:00:00Z AUSTREGÉSILO FILHO, Péricles Tavares http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9693 2024-07-24T20:06:18Z 2024-02-06T00:00:00Z

Título: Avaliação fitoquímica e da atividade antimicrobiana dos extratos hidroalcóólico e hexânico das folhas de Melaleuca leucadendra (Myrtaceae) em Staphylococcus aureus isolados de mastite bovina Autor: AUSTREGÉSILO FILHO, Péricles Tavares Primeiro orientador: MOTA, Rinaldo Aparecido Abstract: The objective of this study was to carry out the phytochemical evaluation and antimicrobial activity of the hydroalcoholic and hexanic extract of the leaves of Melaleuca leucadendra (Myrtaceae), at a concentration of 20mg/mL, on Staphylococcus aureus isolated from bovine mastitis. After plant identification, extracts were prepared and subjected to phytochemical and antibacterial tests. Verification of the presence of metabolite classes was carried out by chromatographic analysis using traditional techniques and verification of compounds by mass spectrometry coupled to electrosplay HPLC-DAD-ESI(+)-MS/MS (micrOTOF-QII) (ESI-MS) ; the hydroalcoholic and hexane extract were analyzed by gas chromatography-mass spectrometry (GC-ME). To evaluate the antibacterial action, the inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were verified in isolates of Staphylococcus aureus from bovine mastitis. The results verified the presence of terpenes, glycosylated flavonoids, aglycone flavonoids, triterpenes, tannin and spectrometry identified 13 chemical superclasses with 88 compounds. In the GC-MS chromatogram, 37 peaks were found, corresponding to 37 chemical compounds. The hydroalcoholic and hexane extracts presented metabolites with biological activity proven in the specialized literature. In turn, the same extracts showed antimicrobial activity against strains of Staphylococcus aureus that cause bovine mastitis, proving to be an alternative in the treatment of the disease. Instituição: Universidade Federal Rural de Pernambuco Tipo do documento: Tese

2024-02-06T00:00:00Z